hoechst nuclear stain Search Results


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ImmunoChemistry Technologies hoechst nuclear stain fluorescence
Zinc depletion enhances ethanol induced apoptosis and cell death through inhibition of autophagy. (A) Annexin V levels in VL17-A cells. (B) Representative images of Annexin V and <t>Hoechst</t> staining in VL17A cells. (I) Zinc depleted cells treated with 100 mM ethanol; (II) cells treated with 5 μM and 100 mM ethanol; (III) zinc depleted cells treated with 100 mM ethanol and CQ and (IV) cells treated with 5 μM of zinc, 100 mM ethanol, and CQ. (C) LDH leakage (percentage of “control group” defined as of cells treated with 5 μM zinc (no ethanol or CQ)). Cells were treated as described in figure 5. Some cells were treated with 30 μM of CQ for 6h. Values are average ± SE.*Significantly different (p < 0.05). Annexin V: interaction zinc vs chloroquine p< 0.0001.
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Cayman Chemical hoechst 33342 nuclear stain
Zinc depletion enhances ethanol induced apoptosis and cell death through inhibition of autophagy. (A) Annexin V levels in VL17-A cells. (B) Representative images of Annexin V and <t>Hoechst</t> staining in VL17A cells. (I) Zinc depleted cells treated with 100 mM ethanol; (II) cells treated with 5 μM and 100 mM ethanol; (III) zinc depleted cells treated with 100 mM ethanol and CQ and (IV) cells treated with 5 μM of zinc, 100 mM ethanol, and CQ. (C) LDH leakage (percentage of “control group” defined as of cells treated with 5 μM zinc (no ethanol or CQ)). Cells were treated as described in figure 5. Some cells were treated with 30 μM of CQ for 6h. Values are average ± SE.*Significantly different (p < 0.05). Annexin V: interaction zinc vs chloroquine p< 0.0001.
Hoechst 33342 Nuclear Stain, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega fluorescent nuclear stain hoechst 33342
Zinc depletion enhances ethanol induced apoptosis and cell death through inhibition of autophagy. (A) Annexin V levels in VL17-A cells. (B) Representative images of Annexin V and <t>Hoechst</t> staining in VL17A cells. (I) Zinc depleted cells treated with 100 mM ethanol; (II) cells treated with 5 μM and 100 mM ethanol; (III) zinc depleted cells treated with 100 mM ethanol and CQ and (IV) cells treated with 5 μM of zinc, 100 mM ethanol, and CQ. (C) LDH leakage (percentage of “control group” defined as of cells treated with 5 μM zinc (no ethanol or CQ)). Cells were treated as described in figure 5. Some cells were treated with 30 μM of CQ for 6h. Values are average ± SE.*Significantly different (p < 0.05). Annexin V: interaction zinc vs chloroquine p< 0.0001.
Fluorescent Nuclear Stain Hoechst 33342, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Zinc depletion enhances ethanol induced apoptosis and cell death through inhibition of autophagy. (A) Annexin V levels in VL17-A cells. (B) Representative images of Annexin V and Hoechst staining in VL17A cells. (I) Zinc depleted cells treated with 100 mM ethanol; (II) cells treated with 5 μM and 100 mM ethanol; (III) zinc depleted cells treated with 100 mM ethanol and CQ and (IV) cells treated with 5 μM of zinc, 100 mM ethanol, and CQ. (C) LDH leakage (percentage of “control group” defined as of cells treated with 5 μM zinc (no ethanol or CQ)). Cells were treated as described in figure 5. Some cells were treated with 30 μM of CQ for 6h. Values are average ± SE.*Significantly different (p < 0.05). Annexin V: interaction zinc vs chloroquine p< 0.0001.

Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

Article Title: Effect of zinc intake on hepatic autophagy during acute alcohol intoxication

doi: 10.1007/s10534-018-0077-7

Figure Lengend Snippet: Zinc depletion enhances ethanol induced apoptosis and cell death through inhibition of autophagy. (A) Annexin V levels in VL17-A cells. (B) Representative images of Annexin V and Hoechst staining in VL17A cells. (I) Zinc depleted cells treated with 100 mM ethanol; (II) cells treated with 5 μM and 100 mM ethanol; (III) zinc depleted cells treated with 100 mM ethanol and CQ and (IV) cells treated with 5 μM of zinc, 100 mM ethanol, and CQ. (C) LDH leakage (percentage of “control group” defined as of cells treated with 5 μM zinc (no ethanol or CQ)). Cells were treated as described in figure 5. Some cells were treated with 30 μM of CQ for 6h. Values are average ± SE.*Significantly different (p < 0.05). Annexin V: interaction zinc vs chloroquine p< 0.0001.

Article Snippet: Values were normalized to Hoechst nuclear stain fluorescence (Immunochemistry Technologies, Bloomington, MN).

Techniques: Inhibition, Staining, Control